In situ Polymerization of a PEG Monomer from the C-Terminus of an Intein Fusion Protein Significantly Improves Pharmacokinetics and Tumor Accumulation
نویسندگان
چکیده
287 ©2011 Society For Biomaterials
منابع مشابه
In situ growth of a PEG-like polymer from the C terminus of an intein fusion protein improves pharmacokinetics and tumor accumulation.
This paper reports a general in situ method to grow a polymer conjugate solely from the C terminus of a recombinant protein. GFP was fused at its C terminus with an intein; cleavage of the intein provided a unique thioester moiety at the C terminus of GFP that was used to install an atom transfer radical polymerization (ATRP) initiator. Subsequent in situ ATRP of oligo(ethylene glycol) methyl e...
متن کاملIn Situ Chemical Oxidative Graft Polymerization of Aniline from Fe3O4 Nanoparticles
This study aims at exploring an effective route in the in situ graft polymerization of aniline from Fe3O4 nanoparticles. To this goal, Fe3O4 magnetic nanoparticles were prepared by coprecipitation method using ammonia solution as the precipitating agent, and were characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM)....
متن کاملFabrication of black printing toner through in situ polymerization: An effective way to increase conversion
Styrene-acrylate copolymer was synthesized in presence of carbon black through heterogeneous polymerization. The percentage of monomer conversion was measured by gravimetric method, also PSA, SEM, DSC and GPC were used for studying the particle size and particle size distribution, morphology, thermal properties and molecular weight, respectively. As well as color properties were characterized b...
متن کاملImmunohistochemical Evaluation of Human p53 Tumor Suppressor Protein Content in Ductal Carcinoma in Situ of the Breast
The focus of this study was to determine if early detection of mutant p53 accumulation may be an early indicator of tumor aggressiveness and transformation to invasive breast cancer. For this purpose, the p53 content of 100 human breast biopsies classified as ductal carcinoma (DCIS), was evaluated by immunohistochemical method. All specimens were microscopically classified into histologic types...
متن کاملIn situ growth of a stoichiometric PEG-like conjugate at a protein's N-terminus with significantly improved pharmacokinetics.
The challenge in the synthesis of protein-polymer conjugates for biological applications is to synthesize a stoichiometric (typically 1:1) conjugate of the protein with a monodisperse polymer, with good retention of protein activity, significantly improved pharmacokinetics and increased bioavailability, and hence improved in vivo efficacy. Here we demonstrate, using myoglobin as an example, a g...
متن کامل